Pixel Count In Histogram

Hi all,

I'm working with a marine imaging lab and have been utilizing Photoshop CC to make area measurements of sediment profile images (SPI) of the sub-seafloor.

Our camera set up utilizes a camera inside of a wedged prism that penetrates the seafloor and takes a photograph of the sedimentary matrix in-situ. The image returned is then manually separated into two layers in photoshop and measured, one layer for the portion of the image penetrating the sediment, and another for the portion of the image showing the water above the seafloor. By selecting each layer and commanding the histogram window to display a pixel count from the selected layer only we establish an area of penetration in out image. Up until today the histogram was giving me a very accurate reading. Today we updated our workflow to utilize 16 bit Adobe RGB images converted to .psd directly from camera raw in 300dpi resolution. Our previous workflow involved converting .raw to .jpeg to .psd in 8 bit, 300dpi resolution. The images look great now but the histogram returns a pixel count of about half of the previously measured values. What is going on here? I can still achieve n accurate pixel count by ctrl clicking my penetration layer and selecting a histogram read from entire image, it still drops a handful of pixels around the marquee but it is much closer to reality.

Any help would be much appreciated.

Cheers,

-Steve



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